PRIME PubMed
Type your tag names separated by a space and hit enter
Abstract
Agarases produce agar oligosaccharides with various structures exhibiting diverse physiological activities. α-Neoagaro-oligosaccharide hydrolase (α-NAOSH) specifically cleaves even-numbered neoagaro-oligosaccharides, producing 3,6-anhydro-l-galactose (l-AHG) and odd-numbered agaro-oligosaccharides (OAOSs). In this study, α-NAOSH from the agar-degrading marine bacterium Gilvimarinus agarilyticus JEA5 (Gaa117) was purified and characterized using an E. coli expression system to produce OAOSs and determine their bioactivity. Recombinant Gaa117 (rGaa117) showed maximum activity at pH 6.0 and 35 °C. rGaa117 retained >80% of its initial activity after 120 min at 30 °C. The activity was enhanced in the presence of Mn[2+]. Km, Vmax, and Kcat/Km values of the enzyme were 22.64 mM, 246.3 U/mg, and 15 s[-1]/mM, respectively. rGaa117 hydrolyzed neoagarobiose, neoagarotetraose, and neoagarohexaose, producing OAOSs that commonly contained l-AHG. Neoagarobiose and neoagarotetraose mixtures, designated NAO24, and mixtures of l-AHG and agarotriose, designated AO13, were obtained using recombinant rGaa16B (β-agarase) and rGaa117, respectively, and their antioxidant activities were compared. AO13 showed higher hydrogen peroxide-scavenging activity than NAO24 in human dermal fibroblasts in vitro because of structural differences: AOSs have d-galactose at the non-reducing end, whereas NAOSs have l-AHG. In conclusion, OAOSs exhibited high ROS-scavenging activity in H2O2-induced human dermal fibroblasts. They may be applicable in cosmetics and pharmaceuticals for prevention of skin aging.
Links
Authors+Show Affiliations
Korea Institute of Ocean Science & Technology (KIOST), Jeju-si 63349, Republic of Korea.
Korea Institute of Ocean Science & Technology (KIOST), Jeju-si 63349, Republic of Korea. Department of Marine Biotechnology, KIOST School, Korea National University of Science and Technology, Daejeon 34113, Republic of Korea.
Korea Institute of Ocean Science & Technology (KIOST), Jeju-si 63349, Republic of Korea. Department of Marine Biotechnology, KIOST School, Korea National University of Science and Technology, Daejeon 34113, Republic of Korea.
Korea Institute of Ocean Science & Technology (KIOST), Jeju-si 63349, Republic of Korea. Department of Marine Biotechnology, KIOST School, Korea National University of Science and Technology, Daejeon 34113, Republic of Korea.
Korea Institute of Ocean Science & Technology (KIOST), Jeju-si 63349, Republic of Korea. Department of Marine Biotechnology, KIOST School, Korea National University of Science and Technology, Daejeon 34113, Republic of Korea.
Korea Institute of Ocean Science & Technology (KIOST), Jeju-si 63349, Republic of Korea.
Korea Institute of Ocean Science & Technology (KIOST), Jeju-si 63349, Republic of Korea. Department of Marine Biotechnology, KIOST School, Korea National University of Science and Technology, Daejeon 34113, Republic of Korea.
Korea Institute of Ocean Science & Technology (KIOST), Jeju-si 63349, Republic of Korea.
MeSH
HumansFibroblastsOligosaccharidesAntioxidantsGlycoside HydrolasesGalactoseSkinAgarDisaccharidesDisaccharidasesGalactosides
Pub Type(s)
Journal Article
Language
eng
PubMed ID
39590775
Citation
Jo, Eunyoung, et al. "Odd-Numbered Agaro-Oligosaccharides Produced By α-Neoagaro-Oligosaccharide Hydrolase Exert Antioxidant Activity in Human Dermal Fibroblasts." Marine Drugs, vol. 22, no. 11, 2024.
Jo E, Gajanayaka ND, Bandara MS, et al. Odd-Numbered Agaro-Oligosaccharides Produced by α-Neoagaro-Oligosaccharide Hydrolase Exert Antioxidant Activity in Human Dermal Fibroblasts. Mar Drugs. 2024;22(11).
Jo, E., Gajanayaka, N. D., Bandara, M. S., Marasinghe, S. D., Park, G. H., Lee, S. J., Oh, C., & Lee, Y. (2024). Odd-Numbered Agaro-Oligosaccharides Produced by α-Neoagaro-Oligosaccharide Hydrolase Exert Antioxidant Activity in Human Dermal Fibroblasts. Marine Drugs, 22(11). https://doi.org/10.3390/md22110495
Jo E, et al. Odd-Numbered Agaro-Oligosaccharides Produced By α-Neoagaro-Oligosaccharide Hydrolase Exert Antioxidant Activity in Human Dermal Fibroblasts. Mar Drugs. 2024 Nov 3;22(11) PubMed PMID: 39590775.
* Article titles in AMA citation format should be in sentence-case
TY - JOURT1 - Odd-Numbered Agaro-Oligosaccharides Produced by α-Neoagaro-Oligosaccharide Hydrolase Exert Antioxidant Activity in Human Dermal Fibroblasts.AU - Jo,Eunyoung,AU - Gajanayaka,Navindu Dinara,AU - Bandara,Minthari Sakethanika,AU - Marasinghe,Svini Dileepa,AU - Park,Gun-Hoo,AU - Lee,Su-Jin,AU - Oh,Chulhong,AU - Lee,Youngdeuk,Y1 - 2024/11/03/PY - 2024/10/04/receivedPY - 2024/10/31/revisedPY - 2024/10/31/acceptedPY - 2024/11/26/medlinePY - 2024/11/26/pubmedPY - 2024/11/26/entrezKW - agar oligosaccharideKW - agaraseKW - antioxidantKW - human dermal fibroblastsJF - Marine drugsJO - Mar DrugsVL - 22IS - 11N2 - Agarases produce agar oligosaccharides with various structures exhibiting diverse physiological activities. α-Neoagaro-oligosaccharide hydrolase (α-NAOSH) specifically cleaves even-numbered neoagaro-oligosaccharides, producing 3,6-anhydro-l-galactose (l-AHG) and odd-numbered agaro-oligosaccharides (OAOSs). In this study, α-NAOSH from the agar-degrading marine bacterium Gilvimarinus agarilyticus JEA5 (Gaa117) was purified and characterized using an E. coli expression system to produce OAOSs and determine their bioactivity. Recombinant Gaa117 (rGaa117) showed maximum activity at pH 6.0 and 35 °C. rGaa117 retained >80% of its initial activity after 120 min at 30 °C. The activity was enhanced in the presence of Mn[2+]. Km, Vmax, and Kcat/Km values of the enzyme were 22.64 mM, 246.3 U/mg, and 15 s[-1]/mM, respectively. rGaa117 hydrolyzed neoagarobiose, neoagarotetraose, and neoagarohexaose, producing OAOSs that commonly contained l-AHG. Neoagarobiose and neoagarotetraose mixtures, designated NAO24, and mixtures of l-AHG and agarotriose, designated AO13, were obtained using recombinant rGaa16B (β-agarase) and rGaa117, respectively, and their antioxidant activities were compared. AO13 showed higher hydrogen peroxide-scavenging activity than NAO24 in human dermal fibroblasts in vitro because of structural differences: AOSs have d-galactose at the non-reducing end, whereas NAOSs have l-AHG. In conclusion, OAOSs exhibited high ROS-scavenging activity in H2O2-induced human dermal fibroblasts. They may be applicable in cosmetics and pharmaceuticals for prevention of skin aging. SN - 1660-3397UR - https://www.unboundmedicine.com/medline/citation/39590775/Odd-Numbered_Agaro-Oligosaccharides_Produced_by_α-Neoagaro-Oligosaccharide_Hydrolase_Exert_Antioxidant_Activity_in_Human_Dermal_Fibroblasts.DB - PRIMEDP - Unbound MedicineER -
